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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Mol Cell Neurosci. 2009 Jun 9;42(2):102–115. doi: 10.1016/j.mcn.2009.06.002

Fig. 6.

Fig. 6

Quantification of β-actin, β-III-tubulin and GAP-43 mRNA in neonatal rat axon-only preparations. Examples of qPCR graphs for β-actin (b), β-III-tubulin (c) and GAP-43 (d). The positive control is shown in plain black, the axon-only preparation in dotted black, and the water control in grey. Quantification relative to coxI (a) showed that β-III-tubulin mRNA levels were about half the levels of β-actin, in both DRGs (**p<0.01) and axons (*p<0.05) (e, f). GAP-43 mRNA, in contrast, was about 25 times lower than actin in DRGs (***p<0.0001), but only 1.5 times lower than actin in axons (e, f). Axonal levels of actin and tubulin were about 1.5% and 1.3% respectively of the DRG levels, whereas, the levels of GAP-43 in axons was 25% of DRG levels (g).