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Differentiation potential of SUCECs into type (I)and II pneumocytes: SUCECs were cultured on collagen 1-coated culture dishes in epithelial differentiation medium for 6 d After the incubation, the cells were examined for the expression of (A) Aqua 5 and (B) SPC, specific markers for type I and II pneumocytes, respectively by IFA. Expression of Aqua 5 and SPC was not observed on SUCECs cultured in DMEM (C and D). Cell nuclei were stained by DAPI.
