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. 2015 Sep 21;112(40):12504–12509. doi: 10.1073/pnas.1509476112

Fig. 5.

Fig. 5.

Interactions among TM, F, and G. (A). TM, F, and G coimmunoprecipitate: F0 can be cleaved to F1 and F2. TM, F, and G were expressed in 293T cells in various combinations. At 24 h.p.t., cells were metabolically labeled for 1 h and treated (+) or untreated (−) with trypsin for 3 h. Cell lysates were immunoprecipitated with anti-F sera, and polypeptides were analyzed by SDS/PAGE. (B) 293T cells expressing combinations of F, G, and TM or PIV5 F, HN, and JPV TM were metabolically labeled as described above, and polypeptides immunoprecipitated. Antibodies were α-FLAG for TM, α-HA for JPV F and G, α-F2 pep for PIV5 F, and monoclonal antibodies HN 1b and HN 4b. F and G were tagged with HA epitope, and TM and TM mutants were tagged with FLAG epitope. MCS, vector of expression plasmid.