Fig. 5.

VemP with the normal ability to arrest its own translation is essential for V. alginolyticus to grow rapidly and induce V.SecDF2. (A) Growth of V. alginolyticus strains with mutations as indicated by numbers (1, WT, squares; 2, ΔsecDF1, diamonds; 3, ΔsecDF2, triangles; 4, Y132amber, crosses) was followed at 30 °C in M63 minimum medium supplemented with either 300 mM Na⁺ (Left) or 33 mM Na⁺ (Right). (B and C) The Vibrio strains used in A were grown in the M63 medium supplemented with 33 mM Na⁺ until an early log phase and then pulse-labeled with [³⁵S]methionine for 30 s. Labeled MBP and V.SecD2 were immunoprecipitated and separated by 10% SDS/PAGE, followed by phosphor imaging. See also Fig. S5.