Table S1.

Conserved features of VemP homologs among Vibrio species

Vibrio species	Hydrophobic	C-terminal sequence of VemP	Residues
V. alginolyticus 138-2	Yes	FYHFTSDHRI SGWKETNAMY VALNSQFSA	159
V. sp. (Ex25)	Yes	FYHFTSDHRI SGWKETNAMY VALNSQFSA	159
V. parahaemolyticus 10329	Yes	FYHFTSDHRI SGWKETNAMY VALNSQFSA	159
V. alginolyticus 12G01	Yes	FYHFTSDHRI SGWKETNAMY VALNSQFSA	159
V. harveyi 1DA3	No	FNHFVSDHRL SGWKETNAMY VALNSQFPA	144
V. sp. EJY3	Yes	FHHFTSDYRL SGWKETNAMY VALNSQFSA	159
V. harveyi (BBA-1116)	Yes (weak)	FNHFVSDHRL SGWKETNAMY VALNSQFSA	159
V. harveyi HY01	Yes (weak)	FNHFVSNHRL SGWKETNAMY VALNSQFSA	159
V. vulnificus (YJ016)	Yes	QYHYVANHRL AGWKETNAMY VALNSQFS	175
V. tubiashii ATCC 19109	Yes (weak)	YNSPYRI SGWKETNALY VALNGQYSVI S	160
V. brasiliensis LMG 20546	Yes (weak)	YTSPHRI AGWKESNALY VALNAHYNSI F	160
V. furnissii (DSM 14383)	Yes	FHPSYRL SGWKETNAMY VALNSQYFH	151
V. coralliilyticus BAA-450	Yes	FTSNHRI SGWKESNALY VALNAQFV	158
V. mimicus MB451 (SX-4)	Yes	FTSTHRL AGWKESNAMY VALNSQYQVS TFKI	156
V. sinaloensis DSM 21326	Yes	FTANHRI SGWKDSNALY VALNSQFA	159
V. anguillarum (68554)	Yes	SNHRI SGWKETNAMY VALNSQY	167
V. orientalis CIP 102891	Yes	RV SGWKESNTLY VALNGHF	155
V. cholerae HC-21A1	Yes	FTSSHRL AGWKESNAMY VALNSQF	155

We compared the VemP homologs among 18 Vibrio species. Most of the deduced VemP amino acid sequences were predicted by the TMHMM program to have a putative signal sequence at the N-terminal region, as indicated by “Yes” (with “weak” indicating relatively low hydrophobicity), whereas V. harvevi 1DA3 may lack the signal sequence. The third column shows the C-terminal segments of VemP, which are highly conserved among Vibrio species. The residues that are identical to those of V. alginolyticus 138-2 are highlighted in boldface.