Table S3.
Plasmids used in this study
| Names | Vectors | Genes on plasmid | Relevant experiment | Source |
| pHM810 | pBAD24 | vemP-secDF2VA | Figs. 4 B, C, E, F, S4 C, D | This study |
| pHM813 | pBAD24 | Δss-vemP-secDF2VA | Figs. 4F, S5A | This study |
| pHM814 | pBAD24 | vemP(Y132amber)-secDF2VA | Fig. 4 B, E | This study |
| pHM829 | pBAD24 | vemP(S158stop)-secDF2VA | Fig. S4 A, C | This study |
| pHM830 | pBAD24 | vemP(F157stop)-secDF2VA | Fig. S4 A, C | This study |
| pHM832 | pBAD24 | vemP(Q156stop)-secDF2VA | Fig. S4 A, C | This study |
| pHM834 | pBAD24 | vemP(S155stop)-secDF2VA | Fig. S4 A, C | This study |
| pHM836 | pBAD24 | vemP(N154stop)-secDF2VA | Fig. S4 A, C | This study |
| pHM838 | pBAD24 | vemP(L153stop)-secDF2VA | Fig. S4 A, C | This study |
| pHM846 | pBAD24 | vemP(W143A)-secDF2VA | Figs. 4C, S4 A, D | This study |
| pHM848 | pBAD24 | vemP(K144A)-secDF2VA | Figs. 4C, S4D | This study |
| pHM915 | pBAD24 | Δss-vemP(132A)-secDF2VA | Fig. 4F | This study |
| pHM934 | pBAD24 | Δss-vemP(S136A)-secDF2VA | Fig. 4F | This study |
| pHM918 | pBAD24 | Δss-vemP(D137A)-secDF2VA | Fig. 4F | This study |
| pHM935 | pBAD24 | Δss-vemP(H138A)-secDF2VA | Fig. 4F | This study |
| pHM899 | pBAD24 | Δss-vemP(R139A)-secDF2VA | Fig. 4F | This study |
| pHM919 | pBAD24 | Δss-vemP(I140A)-secDF2VA | Fig. 4F | This study |
| pHM900 | pBAD24 | Δss-vemP(S141A)-secDF2VA | Fig. 4F | This study |
| pHM920 | pBAD24 | Δss-vemP(G142A)-secDF2VA | Fig. 4F | This study |
| pHM902 | pBAD24 | Δss-vemP(W143A)-secDF2VA | Fig. 4F | This study |
| pHM904 | pBAD24 | Δss-vemP(K144A)-secDF2VA | Fig. 4F | This study |
| pHM921 | pBAD24 | Δss-vemP(E145A)-secDF2VA | Fig. 4F | This study |
| pHM923 | pBAD24 | Δss-vemP(T146A)-secDF2VA | Fig. 4F | This study |
| pHM906 | pBAD24 | Δss-vemP(N147A)-secDF2VA | Fig. 4F | This study |
| pHM925 | pBAD24 | Δss-vemP(A148S)-secDF2VA | Fig. 4F | This study |
| pHM927 | pBAD24 | Δss-vemP(M149A)-secDF2VA | Fig. 4F | This study |
| pHM908 | pBAD24 | Δss-vemP(Y150A)-secDF2VA | Fig. 4F | This study |
| pHM910 | pBAD24 | Δss-vemP(V151A)-secDF2VA | Fig. 4F | This study |
| pHM929 | pBAD24 | Δss-vemP(A152S)-secDF2VA | Fig. 4F | This study |
| pHM912 | pBAD24 | Δss-vemP(L153A)-secDF2VA | Fig. 4F | This study |
| pHM937 | pBAD24 | Δss-vemP(N154A)-secDF2VA | Fig. 4F | This study |
| pHM931 | pBAD24 | Δss-vemP(S155A)-secDF2VA | Fig. 4F | This study |
| pHM939 | pBAD24 | Δss-vemP(Q156A)-secDF2VA | Fig. 4F | This study |
| pHM997 | pTV119N | vemP-secDF2VA | Fig. 4 A, D | This study |
| pHM1023 | pTV119N | Δss-vemP-secDF2VA | Fig. 4 A, D | This study |
| pHM1002 | pTV119N | vemP(Y132amber)-secDF2VA | Fig. 4 A, D | This study |
| pHM1001 | pTV119N | secDF2VA | Fig. 4 A, D | This study |
| pHM1025 | pSTV28 | vemP-secDF2VA | Fig. S5D | This study |
| pHM1027 | pSTV28 | vemP(Y132amber)-secDF2VA | Fig. S5D | This study |
| pHM1033 | pTWV228 | vemP | Fig. S5D | This study |
| pHM1054 | pSTV28 | vemP-(36 bases ins.)-secDF2VA | Fig. 6B | This study |
| pHM1055 | pSTV28 | vemP-(18 bases ins.)-secDF2VA | Fig. 6B | This study |
| pHM1057 | pBAD24 | Δss-vemP-(18 bases ins.)-secDF2VA | Fig. 6C | This study |
| pHM1058 | pBAD24 | Δss-vemP-(36 bases ins.)-secDF2VA | Fig. 6C | This study |
| pHM754 | pBAD24 | secD1VA(his)6 | Figs. 1B, S2A | 5 |
| pHM756 | pBAD33 | secF1VA | Figs. 1B, S2A | 5 |
| pHM753 | pSTV28 | ha-.secDF2VA | Figs. 1B, S2 A, B | This study |
| pGK02 | pSTV28 | ha-secDFEC | Figs. 1B, S2A | 5 |
| pHM802 | pBAD24 | secD1VA internal (his)10-GT-(his)10 | Fig. S2B | This study |
| pHM769 | pBAD24 | secDF2VA | Fig. S3C | This study |
| pHM1014 | pBAD24 | secDF2(R232M)VA | Fig. S3C | This study |
| pHM748 | pBAD24 | secDFEC(his)6 | Fig. S3C | This study |
| pST30 | pACYC | syd | Fig. S5C | 49 |
| pEI7 | pMMB206 | secDF2VA | Figs. 2D, S5E | This study |
| pEI8 | pMMB206 | secDF2(R232M)VA | Figs. 2D, S5E | This study |
| pHM775 | pTH100 | secD2VA 5′-kan-secF2VA 3' | Fig. S2D | This study |
| pNR5 | pTH100 | secD1VA 5′-kan-secF1VA 3' | Fig. S2D | This study |
| pEI9 | pSW7848 | vemP(Y132amber)-secD2VA | Fig. 5 | This study |
| pBAD24 | 70 | |||
| pBAD33 | 70 | |||
| pTV119N | Takara Bio | |||
| pTWV228 | Takara Bio | |||
| pSTV28 | Takara Bio | |||
| pFLAG-CTC | lacIq | Sigma-Aldrich | ||
| pCH85 | pTWV228 | ha-tag | 56 | |
| pTH100 | 54 | |||
| pSW7848 | 57 | |||
| pMMB206 | 71 |