Table S1.

Typical MVID features found in our MVID mouse model were compared with a newly identified MYO5B mutant MVID patient

MVID characteristics	MVID mouse model	Novel MVID patient
Apical microvilli	Erosion to complete loss	Erosion to complete loss
Microvillus inclusions	∼20% of villus enterocytes	Frequent in villus enterocytes
Basolateral microvilli	Yes	Yes
Subapical vesicles/secretory granules	Translucent in villus, electron dense in crypt	Translucent, electron dense in crypt
PAS/ALP	Subapical	Subapical
RAB11A	Subapical	Subapical
RAB8A	Almost absent, more diffuse in cytoplasm	n.a.
LAMP1	Almost absent, more diffuse in cytoplasm	More diffuse in cytoplasm
Ezrin/pERM/Actin	Cytoplasmic inclusions	Cytoplasmic inclusions
STX3	More diffuse in cytoplasm; cytoplasmic inclusions	More diffuse in cytoplasm; cytoplasmic inclusions
E-cadherin	Basolateral and apical	Basolateral and apical
Na/K-ATPase	Basolateral and apical	Basolateral and apical