Fig. 1.

AA enrichment leads to reduced PG-G levels in zymosan-stimulated RAW264.7 cells. Cells (5 × 10⁵) were enriched with AA, incubated with LPS and IFN-γ to induce COX-2 expression, and then stimulated with zymosan to trigger release of AA (A) and 2-AG (B) and biosynthesis of PGs (C) and PG-Gs (D). Data are the mean ± SD of four independent experiments, each performed in triplicate. Statistical significance was determined by Student’s t test (*P < 0.05, **P < 0.01, ****P < 0.0001 compared with unenriched).