Fig. 4.
COX-2 reaction model (CORM) for the interaction of substrates AA and 2-AG with COX-2, a functional heterodimer comprising a catalytic subunit (Left, green) and an allosteric subunit (Right, red). The model provides values for the equilibrium constants of all intermediate complexes and rate constants for product-forming steps as indicated. Constants depicted in red were fixed to experimentally determined values. Specifically, Km values, which varied little between experiments, were based on the data in Fig. S3. To optimize fitting to the data in Fig. 3, kcat values obtained from those data were used. Values depicted in blue are the most probable values for those parameters, based upon computational results.