Fig. S5.

Analysis of the effects of AA on 2-AG (0.5, 1, 2, 4, 8, and 16 μM; A–F, respectively) oxygenation using classical models of inhibition. The model incorporated classical uncompetitive inhibition based on binding of AA to the allosteric site of COX-2 along with competition between the two substrates for the catalytic site and substrate inhibition of 2-AG. The data points are values obtained in the experiment described in the legend to Fig. 3, and the curves in red are the result of nonlinear regression analysis performed using GraphPad Prism 6 software.