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. 2015 Sep 23;112(40):E5461–E5470. doi: 10.1073/pnas.1508449112

Fig. 5.

Fig. 5.

Sgf29 promotes processive acetylation against H3K4me3 nucleosomes. (A) Schematic of the competition assay to test for processive behavior. (B–H) Results from competition assays containing: the wild-type HAT module and unmodified nucleosomes (B), the wild-type HAT module and H3K4me3 nucleosomes (C), the wild-type HAT module, H3K4me3 nucleosomes, and H3K4me3 peptide (amino acids 1–8) added at the beginning of the reaction (D), the ΔTudor HAT module and unmodified nucleosomes (E), the ΔTudor HAT module and H3K4me3 nucleosomes (F), the Δbromodomain HAT module and unmodified nucleosomes (G), or the Δbromodomain HAT module and H3K4me3 nucleosomes (H). (Upper Row) Acid–urea gel of competition assay visualized using a Typhoon scanner. Background subtraction was performed using a sliding paraboloid in ImageJ. (Lower Row) Quantitation of acid–urea gel in ImageJ.