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. 2015 Sep 23;112(40):E5461–E5470. doi: 10.1073/pnas.1508449112

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Fig. S7. — Alexa Fluor dyes do not bias HAT module activity. (A and B) Fluorescent images of HAT reactions resolved on acid–urea gels containing the wild-type HAT module and 1 μM nucleosomes labeled with Alexa Fluor 647 (A) or Alexa Fluor 488 (B). (C and D) Fluorescent images of HAT reactions resolved on acid–urea gels containing mixtures of differentially labeled but otherwise unmodified nucleosomes at 1:1 (C) or 4:1 (D) molar ratios. (Left) Merged image with Alexa Fluor 488-labeled nucleosomes colored green and Alexa Fluor 647-labeled nucleosomes colored red. (Right) Individual images used to generate the merged image.