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The bromodomain of Gcn5 facilitates acetylation on nucleosomal substrates. (A) HAT reactions using the Δbromodomain HAT module and 200 nM fluorescently labeled nucleosomes resolved on an acid–urea gel. (B) HAT reactions using the Δbromodomain HAT module and 200 nM fluorescently labeled H3K4me3 nucleosomes resolved on an acid–urea gel.
