Table 1.

Summary of steady-state kinetic parameters fit from titrations in which the concentration of the histone H3 amino acids 1–21 peptide was varied and the acetyl-CoA concentration was held constant at 100 μM

HAT module	Histone H3 peptide amino acids 1–21	kcat, s−1	Km, μM	kcat/Km, μM−1⋅s−1
Wild type	Unmod	2.5 ± 0.1	250 ± 40	0.010 ± 0.002
ΔTudor	Unmod	2.5 ± 0.1	210 ± 30	0.012 ± 0.002
Sgf29 T202A/Y205V	Unmod	3.4 ± 0.2	320 ± 50	0.011 ± 0.002
ΔBromodomain	Unmod	1.8 ± 0.07	380 ± 40	0.0047 ± 0.001
Wild type	H3K4me3	2.4 ± 0.08	77 ± 10	0.031 ± 0.004
ΔTudor	H3K4me3	2.5 ± 0.09	190 ± 20	0.013 ± 0.001