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Immunoblot analysis of YTH clones. (A–C) Expression of the YTH clones shown in Fig. 1. Yeast total extracts were prepared from transformants shown in Fig. 1F (A), Fig. 1G, Left (B), and Fig. 1G Right (C) and subjected to immunoblotting using an anti-GBD antibody to detect GBD-fusion proteins and an anti-HA antibody to detect the GAD-HA-fusion proteins.
