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ALIX does not affect AMSH3 DUB activity. DUB assay with K63-linked diubiquitin-TAMRA. Recombinant AMSH3 was incubated with diubiquitin-TAMRA with or without preincubation with an equimolar amount of ALIX. Fluorescence was measured every minute. Black, diubiquitin-TAMRA with AMSH3; Red, diubiquitin-TAMRA with AMSH3 and ALIX; Gray, diubiquitin-TAMRA only.
