Table 1.

Design success rate

Structure	Designs tested	Expressed*	Soluble*	αβ-protein CD spectrum	Monomeric†	Well-resolved HSQC	Success (%)‡
Fd_5S	6	6	6	0	3	0	0 (0)
Fd_5A	12	12	12	6	9	4	4 (33)
Fd_7S	10	10	8	6	7	1	1 (10)
Fd_7A	11	9	8	6	3	3	2 (18)
Fd_9A	12	12	11	11	7	3	3 (25)
Rsmn2x2_5	9	9	7	8	6	2	2 (22)
Rsmn2x2_6	12	12	12	10	4	4	4 (33)

The second column shows the number of designs experimentally tested for the backbone blueprints (Fig. 4) indicated in the leftmost column. The subsequent columns give the number of designs that satisfy each criterion.

^*Expression and solubility were assessed by SDS/PAGE and mass spectrometry.

^†SEC-MALS was used to determine oligomerization state.

^‡The successful designs are defined as those that satisfy all criteria. The details of the results are shown in SI Appendix, Tables S4–S8.