Mutations in POL2A cause reduced fertility and meiotic defects in Arabidopsis. (A) Alexander red staining of WT, pol2a-1, and pol2a-2 anthers. (B) Tetrad observation of the WT, pol2a-1, and pol2a-2. (C) A histogram of tetrads vs. polyads in the WT and mutants. (D) Chromosome morphologies of the WT, pol2a-1, and pol2a-2 by DAPI staining. Arrows indicate probable nonhomologous associations. (Scale bars: A, 100 μm; B, 10 μm; D, 5 μm.)