Fig. 4.

POL2A is not required for DSB formation but promotes progression of meiotic recombination. (A) Immunostaining with γ-H2AX (red), RAD51 (green), and DAPI counterstain (blue) at zygotene and pachytene in the WT and pol2a-1. (Scale bar: 5 μm.) (B) Variation of the γ-H2AX and RAD51 foci numbers above shown by boxplot in the WT and pol2a-1. **P ≤ 0.01 with a one-tailed Student t test. (C) Percentages of leptotene, zygotene, and pachytene cells at ZT4 (4 h after lights on), ZT5, ZT6, and ZT7, with error bars from analyses of three to five biological replicates in the WT, pol2a-1, and pol2a-2.