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. 2015 Sep 22;112(40):12432–12437. doi: 10.1073/pnas.1508651112

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Fig. S8. — Main stages of suspensor development after laser ablation and the putative mechanism for suspensor cell-fate transition. (A) The eight-celled embryo stage. The auxin accumulates in the embryo proper during this stage (the green area). (B) The eight-celled embryo after ablation. The auxin accumulates in the uppermost cell of the suspensor. The arrows indicate the direction of auxin transport from the suspensor to the top of the suspensor. (C) The ablated eight-celled embryo after culture. The suspensor cell generates the second embryo with similar auxin distribution. (D) The 32-celled embryo stage. Auxin start to transport downward. (E) The 32-celled embryo stage after microdissection. The arrows show the orientation of auxin transport; the auxin accumulates in the uppermost cell of the suspensor. (F) The suspensor cell develops into a second embryo after ablation. (G) The heart-stage embryo. (H) The heart-stage embryo after microdissection. (I) The suspensor does not develop any further after microdissection, but the embryo develops well.