Fig. 1.

lnx2a is expressed in the ventral pancreas during early pancreas specification. Lateral views (A and I), dorsal views (B–E), and ventral views (F–H). (A) lnx2a transcripts (blue) are detected in regions of the endoderm (arrow), forebrain (arrowhead), and the spinal cord at 24 hpf. Expression of pdx1 (B, D, and G), ins (C, E, and I) and foxa3 (F) are in red. lnx2a expression is observed in the antero-ventral part of pdx1-positive pancreas precursors at 21 (B) and 26 (D) hpf, but excluded from β-cells (ins⁺) in the dorsal pancreas at 21 (C), 26 (E), and 48 (I) hpf. At 48 hpf, lnx2a expression is detected in the ventral pancreas but not in the intestine, swim bladder, or liver (F–H). SB, swim bladder; In, intestine; Li, liver; Pa, pancreas; VP, ventral pancreas. (Scale bars: 50 μm.)