Fig. 4.

The lnx2a^Δ329 mutant recapitulates the morphant phenotype. (A) Sequence of the lnx2a^Δ329 mutation (Fig. 3A). The lowercase sequence in the mutant indicates that it is in intron 2, with lnx2a^Δ329 having lost the exon 2 splice donor site. (B and C) Genotyping of lnx2a^Δ329 by genomic PCR (Δ329), RT-PCR (Δ510 equaling exon 2) (B), and by Western blotting of embryo extracts showing the presence of N-truncated protein (C). (D) Phenotypic analysis of lnx2a^Δ329 mutants at 48 hpf. (Scale bar: 50 μm.) (E) The quantification of pancreatic defects by analysis of ptf1a expression, classified as in Fig. 2D. Development of the exocrine pancreas is specifically suppressed in lnx2a^Δ329.