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Cognate iNKT-cell help induces more TFR cells than noncognate iNKT-cell help. Splenocytes from C57BL/6 mice immunized with vehicle [PBS/0.1% (wt/vol) BSA], 100 μg NP-KLH plus 0.5 μg αGalCer (αGC), or 0.5 μg NPαGalCer (NPαGC) were assessed at day 6 by FACS analysis for (A) numbers or (B) percentages of B220−TcRβ+CD4+CXCR5+PD-1+FoxP3+ TFR cells (representative of three experiments; n = 4 mice per group). *P ≤ 0.05.
