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. 2015 Sep 21;112(40):12474–12479. doi: 10.1073/pnas.1504790112

Fig. 3.

Fig. 3.

Selective elimination of PD-1 on iNKT cells increases CXCR5+Bcl6+ iNKTFH cells but does not rescue humoral memory. Eight days after i.v. immunization of iNKT PD-1 KO or iNKT WT mixed BM chimeras with 0.5 μg NPαGalCer (NPαGC) or 100 μg NP-KLH plus 0.5 μg αGalCer (αGC), splenocytes were labeled for FACS to determine numbers of (A) CXCR5+PD-1+ iNKT cells, (B and C) CXCR5+Bcl6+ iNKT cells, (D and E) CXCR5+PD-1+Bcl6+ T cells, and (F) NP-specific germinal center B cells (representative of three experiments; n = 5 mice per group). *P ≤ 0.05. (G) ELISA also detected 4-hydroxy-5-iodo-3-NP (NIP) -specific IgG in sera from iNKT PD-1 KO or iNKT WT mixed BM chimeras immunized i.v. with 100 μg NP-KLH plus 0.5 μg αGC or 0.5 μg NPαGC on day 0 followed by an i.v. boost of 100 μg NP-KLH or 0.5 μg NPαGC on day 50 (representative of two experiments; n = 4–6 mice per group). *P ≤ 0.05.