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. 2015 Sep 21;112(40):12474–12479. doi: 10.1073/pnas.1504790112

Fig. S4.

Fig. S4.

Selective elimination of CD25 on iNKT cells increases iNKTFH cells but does not rescue humoral memory. Twelve days after i.v. immunization of iNKT CD25KO or iNKT WT mixed BM chimeras with 0.5 μg NPαGalCer (NPαGC) or 100 μg NP-KLH plus 0.5 μg αGalCer (αGC), splenocytes were labeled for FACS to determine numbers of (A) iNKT and (B) iNKTFH cells (representative of two experiments; n = 5 mice per group in duplicate). *P ≤ 0.05. (C) ELISA also detected NIP-specific IgG in sera from iNKT CD25KO or iNKT WT mixed BM chimeras immunized i.v. with 100 μg NP-KLH plus 0.5 μg αGC or 0.5 μg NPαGC on day 0 followed by an i.v. boost of 100 μg NP-KLH or 0.5 μg NPαGC on day 47. Sera was collected 14 d after the secondary boost (representative of two experiments; n = 4 mice per group).