Table 2.
Enzymatic profile of B. hyodysenteriae type strain (B78T) and six strains of B. suanatina
| Strain | Brachyspira panel a | api-ZYM enzyme activitiesb | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Haem | IS | Hipp | α-gal | β-glu | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
| B78T | s | + | – | – | + | 0 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 1 | 1 | 5 | 2 | 4 | 4 | 0 | 0 | 0 |
| AN4859/03 | s | + | – | – | + | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 5 | 0 | 2 | 4 | 0 | 0 | 0 |
| AN1681:1/04 | s | + | – | – | + | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 4 | 0 | 2 | 3 | 0 | 0 | 0 |
| AN2384/04 | s | + | – | – | + | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 5 | 0 | 2 | 4 | 0 | 0 | 0 |
| Dk12570-2 | s | + | – | – | + | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 5 | 0 | 2 | 3 | 0 | 0 | 0 |
| AN3949:2/02 | s | + | – | – | + | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 5 | 0 | 2 | 3 | 0 | 0 | 0 |
| AN1418:2/01 | s | + | – | – | + | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 5 | 0 | 3 | 4 | 0 | 0 | 0 |
a Brachyspira panel according to Fellström et al., 1999. Results according to Råsbäck et al., 2007. Haem = intensity of β-haemolysis; IS = indole spot test; Hipp = hippurate cleavage capacity; α-galactosidase; β-glucosidase; s = strong reaction; + = positive reaction, − = negative reaction
bAPI-ZYM test: 1 control; 2 alkaline phosphatase; 3 C4 esterase; 4 C8 esterase lipase; 5 C14 lipase; 6 leucine arylamidase; 7 valine arylamidase; 8 cystine arylamidase; 9 trypsin; 10 chymotrypsin; 11 acid phosphatase; 12 naphtolphosphohydrolase; 13 α-galactosidase; 14 β-galactosidase; 15 β-glucoronidase; 16 α-glucosidase; 17 β-glucosidase; 18 N-acetyl-β-glucosaminidase; 19 α-mannosidase; 20 α-fucosidase; values 0–5 assigned according to the manufacturer’s instruction 0 = negative reaction, 5 = maximum intensity