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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Biochem J. 2009 Nov 11;424(2):273–283. doi: 10.1042/BJ20090699

Figure 4. Effect of simultaneous down-regulation of CerS2 and individual ceramidases on LC and VLC ceramides.

Figure 4

SMS-KCNR neuroblastoma cells were treated with 30 nM control siRNA, 15 nM CerS2 siRNA alone or in combination with 15 nM alkaline ceramidase 1 (alkCDase1) siRNA, 15 nM alkaline ceramidease 2 (alkCDase2) siRNA or 15 nM acid ceramidase (aCDase) siRNA for 48 h. Lipids were extracted from the cell pellets and subjected to MS analysis. Sphingolipid levels were normalized to cellular lipid phosphate (pmol/nmol of phosphate) and are presented as a fold-change over control. The error bars represent the range for two independent experiments. (A) C16 ceramide and (B) C24:1 ceramide.