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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Biochem J. 2009 Nov 11;424(2):273–283. doi: 10.1042/BJ20090699

Figure 8. Down-regulation of CerS2 resulted in activation of the UPR.

Figure 8

(A) Western blot analyses with phospho- and total eIF2α antibodies on lysates from SMS-KCNR cells treated with 20 nM control and two different CerS2 siRNAs oligonucleotides for 72 h. Values are means±S.E.M. from three independent experiments. (B) Western blot analyses with phospho- and total eIF2α antibodies on lysates from MCF-7 cells treated with 20 nM control and CerS2 siRNA for 72 h. Values are means±S.E.M. from three independent experiments. The ImageJ program was used for quantification of phospho- and total eIF2α bands. *P < 0.02 in (A) or *P < 0.05 in (B); **P < 0.01 (compared with control siRNA; as measured using a Student’s t test; SigmaPlot). (C and D) RT-PCR results with primers specific for CHOP (C) or sXBP-1 (D) on RNA samples isolated from SMS-KCNR cells treated with 20 nM control and two different CerS2 siRNA oligonucleotides for 72 h. Values are means±S.E.M. from four independent experiments. *P < 0.02 and ** P < 0.01 (compared with control siRNA; as measured using a Student’s t test; SigmaPlot).