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. 2015 Oct 12;11:46. doi: 10.1186/s13007-015-0090-6

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© Kumar and Turner. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of swelling time on sample colour. 13 tubes containing increasing amounts of cell wall derived glucose are shown. Acetic nitric extracted stem pieces were swelled in 67 % sulphuric acid and left on the bench. Photographs were taken 6 h, 1 day and 6 days after adding sulphuric acid. Normally, we carry out the anthrone assays within 8 h. The error bars are standard error of mean (SEM)