Table 1 .

Troubleshooting guide

Steps	Problem	Possible reason	Solution
20	Anthrone solution not developing colour	Used 67 % sulphuric acid instead of conc. Sulphuric acid	Remake anthrone solution with conc. Sulphuric acid
20	Anthrone solution getting dark too quickly	Used sulphuric acid from an old bottle	Remake anthrone solution with conc. Sulphuric acid from a new bottle
23	OD620 values outside the standard curve range	Too much/too little sample used for anthrone assay	Use more/less amount of sulphuric acid treated sample