Table 3. Replication and transmission in chickens of pH1N1-H9N2 “7+1” reassortants.
| Virus | Inoculated chickens | Contact chickens | |||||
|---|---|---|---|---|---|---|---|
| 3dpi | 5dpi | lung | Seroconversion | 2dpc | 4dpc | Seroconversion | |
| HB08 | 10/10 (4.6 ± 0.8)a | 6/7 (3.9 ± 0.2) | 3/3 (4.9 ± 0.3) | 7/7 (1024–2048)b | 2/5 (3.7 ± 0.2) | 5/5 (3.8 ± 0.3) | 5/5 (256–1028) |
| BJ09 | 0/10 | 0/7 | 0/3 | 0/7 | 0/5 | 0/5 | 0/5 |
| BJ09-PB2:HB08 | 9/10 (3.0 ± 0.4) | 2/7 (2.5 ± 0.1) | 1/3 (3.3) | 7/7 (512–2048) | 0/5 | 1/5 (1.8) | 2/5 (256–1024) |
| BJ09-PB1:HB08 | 10/10 (4.3 ± 0.7) | 3/7 (3.6 ± 0.1) | 3/3 (5.0 ± 1.8) | 7/7 (512–1024) | 3/5 (3.5 ± 0.5) | 3/5 (3.8 ± 0.4) | 5/5 (256–1280) |
| BJ09-PA:HB08 | 9/10 (4.0 ± 0.6) | 2/7 (3.8 ± 0) | 2/3 (4.4 ± 0.2) | 7/7 (1024–2048) | 1/5 (1.8) | 3/5 (2.4 ± 0.4) | 4/5 (128–1024) |
| BJ09-HA:HB08 | 1/10 (1.8) | 0/7 | 1/3 (1.8) | 6/7 (1024–2048) | 0/5 | 1/5 (1.8) | 2/5 (256-512) |
| BJ09-NP:HB08 | 9/10 (4.0 ± 0.5) | 2/7 (3.6 ± 0.3) | 2/3 (4.5 ± 1.8) | 7/7 (512-1024) | 1/5 (1.8) | 3/5 (1.8 ± 0) | 3/5 (128-512) |
| BJ09-NA:HB08 | 10/10 (5.0 ± 1.1) | 7/7 (4.0 ± 0.6) | 3/3 (4.9 ± 0.8) | 7/7 (1024-2048) | 4/5 (3.7 ± 0.2) | 5/5 (4.0 ± 0.4) | 5/5 (256-2048) |
| BJ09-M:HB08 | 9/10 (4.6 ± 0.8) | 5/7 (4.1 ± 0.8) | 3/3 (4.8 ± 1.5) | 7/7 (1024-2048) | 3/5 (2.7 ± 0.2) | 5/5 (3.6 ± 0.1) | 5/5 (128-2048) |
| BJ09-NS:HB08 | 8/10 (4.3 ± 0.7) | 5/7 (3.6 ± 0.3) | 3/3 (4.0 ± 1.1) | 7/7 (1024-2048) | 3/5 (2.8 ± 0.3) | 5/5 (3.5 ± 0.1) | 5/5 (256–2048) |
| HB08-PB2:BJ09 | NDc | ND | ND | ND | ND | ND | ND |
| HB08-PB1:BJ09 | 3/10 (2.5 ± 0.3) | 0/7 | 0/3 | 7/7 (512–2048) | 0/5 | 2/5 (1.9 ± 0.1) | 3/5 (256–1024) |
| HB08-PA:BJ09 | 2/10 (2.1 ± 0.4) | 0/7 | 1/3 (1.8) | 7/7 (512–1024) | 0/5 | 0/5 | 2/5 (128–512) |
| HB08-HA:BJ09 | 3/10 (1.8 ± 0) | 0/7 | 0/3 | 7/7 (256–1024) | 0/5 | 0/5 | 0/5 |
| HB08-NP:BJ09 | 4/10 (2.3 ± 0.3) | 0/7 | 0/3 | 7/7 (1024) | 0/5 | 1/5 (1.8) | 3/5 (256–1024) |
| HB08-NA:BJ09 | 4/10 (2.7 ± 0.3) | 0/7 | 0/3 | 7/7 (512–1024) | 0/5 | 2/5 (1.9 ± 0.1) | 5/5 (128–512) |
| HB08-M:BJ09 | 0/10 | 0/7 | 0/3 | 2/7 (128–512) | 0/5 | 0/5 | 0/5 |
| HB08-NS:BJ09 | 3/10 (2.6 ± 0.4) | 0/7 | 0/3 | 7/7 (1024–2048) | 1/5 (1.8) | 2/5 (1.9 ± 0.1) | 5/5 (512–1024) |
aNumber of positive oropharyngeal swabs/total number (mean titers [log10 EID50/mL] ± SD). Serum was collected on 21 dpi, and BJ09 or HB08 viruses were used in HI assays.
bNumber of positive chickens/total number (rang of HI titers)
cND, not done.