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Primers and restriction enzyme used for analysis of these three SNPS
| SNPs | Primers (5′–3′) | Products (bp) | Restriction enzyme |
|---|---|---|---|
| hsa-mir-196a2 rs11614913 C/T | F: CCCCTTCCCTTCTCCTCCAGATA | 149 | MspI |
| R: CGAAAACCGACTGATGTAACTCCG | |||
| hsa-mir-499 rs3746444 A/G | F: CAAAGTCTTCACTTCCCTGCCA | 146 | BclI |
| R: GATGTTTAACTCCTCTCCACGTGATC | |||
| hsa-mir-146a rs2910164 C/G | F: CATGGGTTGTGTCAGTGTCAGAGCT | 147 | SacI |
| R: TGCCTTCTGTCTCCAGTCTTCCAA |
