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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Mol Microbiol. 2013 Sep 8;90(2):383–399. doi: 10.1111/mmi.12371

Fig. 1.

Fig. 1

Conserved residues within the N-terminal region of COT1 affect fungal morphology.

A. Schematic summary of amino acid alterations introduced into COT1 NTR. The two highly conserved phosphorylation sites at the kinase domain (Ser417) and C Terminal domain (Thr589) are also shown. Grey box represents the NTR and a dotted box represents the kinase domain. The residues’ numbers refer to the COT1 protein (long transcript) without the Myc tag.

B. Growth of myc-cot-1 and the NTR mutants harbouring the different Myc-tagged alleles of cot-1. Cultures were grown overnight at 34°C.

C. Hyphal morphology at the edge of the colony of wild-type, cot-1(ts), myc-cot-1 and the NTR point-mutation strains cultured overnight at 34°C. Bar = 100 μm.

D. Microscopic images of myc-cot-1, cot-1(R167A) and cot-1(R203A) hyphal tips grown at 34°C. Bar represents 10 μm.