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Mutating COT1 Arg203 to Ala impairs COT1-MOB2A interactions: Growth curves of yeast harbouring the indicated two-hybrid plasmids in SD-Leu/-Trp liquid medium (black) or in SD high selection liquid medium -Leu/-Trp/-His/-Ade (white). Similar growth of the yeast strains in the two different media indicates strong physical interaction between the two proteins.
