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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Mol Microbiol. 2013 Sep 8;90(2):383–399. doi: 10.1111/mmi.12371

Fig. 7.

Fig. 7

Mutations at the COT1 NTR affect COT1 dimerization as determined by Yeast Two Hybrid assay. Yeast transformants carrying the each of COT1 alleles, both as bait and as pray, were grown overnight in SD medium lacking Leu and Trp. The supernatant of the yeast culture was assayed for α-galactosidase activity. High α-galactosidase activity indicates strong interaction between the tested proteins. The results are an average of three independent experiments that were performed in triplicate. Bar indicates standard error.