Table 1.
Morphological consequences of mutations within the COT1 NTR-encoding region.
| Developmental process | myc-cot-1 | cot-1(R167A) | cot-1(S189A) | cot-1(S189E) | cot-1(E198A) | cot-1(R203A) |
|---|---|---|---|---|---|---|
| Hyphal growth rate (mm h−1) | 4.99 ± 0.05 | 4.81 ± 0.03 | 4.55 ± 0.05 | 4.62 ± 0.04 | 1.75 ± 0.07 | 3.79 ± 0.04 |
| Length between branches (μm) | 169 ± 4 | 166 ± 4 | 147 ± 5 | 180 ±9 | 71 ± 1 | 143 ± 3 |
| Hyphal width (μm) | 11 ± 3.6 | 11.5 ± 4.0 | 11.5 ± 2.9 | 13.0 ± 4.6 | 5.1 ± 2.1 | 9.4 ± 3.0 |
| Total cfu (% of myc-cot-1) | 100% ± 4% | 69% ± 4% | 103% ± 8% | 136% ± 8% | 34% ± 6% | 52% ± 3% |
| Germinating conidia after 4 h of incubation (% of total) |
72% ± 0.5% | 72% ± 0.4% | 37% ± 1.1% | 45% ± 2.4% | 42% ± 4.2% | 71% ± 4.6% |