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. Author manuscript; available in PMC: 2016 May 6.
Published in final edited form as: Neuron. 2015 Apr 23;86(3):680–695. doi: 10.1016/j.neuron.2015.04.003

Figure 8. SB216763 treatment restores axon branch numbers.

Figure 8

(A, B) Western blot analysis of lysates of prefrontal cortex from Zdhhc8-deficient mice at 8 weeks demonstrating reduced levels of pAKT(S473) and pGSK3β(S9).

(C, D)Western blot analysis of lysates of prefrontal cortex from Df16(A)+/− mice at 8 weeks demonstrating reduced levels of pAKT(S473) and pGSK3β(S9).

(E) Representative images of ipsilateral axon branching of EGFP–labeled neurons from coronal sections of SB216763-treated brain at P7.5.

(F) Traces of axon branches in the ipsilateral cortical layers 2–4 of coronal sections from P7.5 brains in utero electroporated with EGFP at E15.5 and treated with DMSO or SB216763 from P2 to P7.

(G) Quantification of axon morphology reveals that SB216763 treatment restored axon branch numbers in the ipsilateral cortical layers 2–4. Scale bar, 20 µm.

(H) Representative images of contralateral axon branching of EGFP–labeled neurons from coronal sections of SB216763-treated brain at P7.5.

(I) Traces of axon branches in the contralateral cortical layers 2–4 of coronal sections from P7.5 brains in utero electroporated with EGFP at E15.5 and treated with DMSO or SB216763 from P2 to P7.

(J) Quantification of axon morphology reveals that SB216763 treatment restored axon branch numbers in the contralateral cortical layers 1–4. Scale bar, 20 µm.

Data are shown as means ± s.e.m. *, P < 0.05; **, P < 0.001; ***, P < 0.0001; Student’s t-test.