Fig. 4.

Analysis of importin-α homologs in S. mediterranea. a Phylogenetic analysis of Importin-α (ΙΜΑ) homologs. Each protein is labeled with Uniprot accession number, gene name, and species. Species abbreviations are as follows: HUMAN = Homo sapiens, PONAB = Pongo abelii, BOVIN = Bos taurus, MOUSE = Mus musculus, DANRE = Danio rerio, RAT = Rattus norvegicus, CHICK = Gallus, YEAST = Saccharomyces cerevisiae, SCHPO = Schizosaccharomyces pombe, DICDI = Dictyostelium discoideum, ARATH = Arabidopsis thaliana, ORYSJ = Oryza sativa subspecies japonica, SOLLC = Solanum lycopersicum, SMED = Schmidtea mediterranea, CAEEL = Caenorhabditis elegans, DROME = Drosophila melanogaster, XENLA = Xenopus laevis. Arrows indicate Smed-ima-1 and Smed-ima-2. b Domain structure of Schmidtea mediterranea Importin-α proteins. IBB = Importin-β binding domain. ARM = Armadillo repeats. c-d Whole-mount in situ hybridization to Smed-ima-1 and Smed-ima-2. The irradiated worms were treated with 60 Gy γ-irradiation 3 days prior to fixation to destroy the stem cells. The regenerating animals were amputated to remove the half of the head oriented toward the bottom of the picture two days prior to fixation. Arrows indicate the blastema. Animals were imaged from the ventral side with anterior to the left. Scale = 0.5 mm