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. 2015 Oct 12;15:682. doi: 10.1186/s12885-015-1706-y

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© Wang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — HeLa cell and HT-29 cell apoptosis after treatment with hesperidin (HES) observed using Hoechst 33342 staining. a and c HeLa cells and HT-29 cells were treated with HES (0, 40, 80, and 160 μM) for 48 h. Apoptotic cells (Arrows) exhibited morphological changes in the nuclei typical of apoptosis. Scale bar = 100 μm. b and d HES-induced increase of apoptosis in HeLa cells and HT-29 cells. Cells were treated with HES at concentrations of 0, 40, 80 and 160 μM for 48 h. The level of apoptosis was analyzed by Hoechst 33342 staining; *p <0.05 versus control group (0 μM) (one-way ANOVA followed by Tukey’s post hoc test)