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. 2015 Oct 15;26(20):3596–3605. doi: 10.1091/mbc.E15-05-0317

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© 2015 Dean and Mitchell. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — ODA10 is restricted to DM1. (A, B) Negative-stain electron micrograph of ODA10HA localized with 10-nm gold particles (arrows) on unfixed axonemes. Bracket in A indicates the region enlarged in the inset. (C, D) Pre-embedding, labeled oda10,ODA10HA axonemes, thin section. (E) Wild-type axoneme (proximal). (F) Enlarged averaged view of wild- type DM1–DM2 bridge. (G) oda10 axoneme (proximal). (H) Enlarged averaged view of oda10 DM1–DM2 bridge. The DM1–DM2 bridge is marked by an asterisk in E and G. Scale bar, 1 μm (A; inset, 100 nm); 200 nm (B–E, G), 50 nm (F, H).