Fig. 3.

Tivantinib induces microtubules depolymerization in HCC cells independent of MET and GSK3. a MHCC97L and Huh7 cells were treated with tivantinib, vincristine or JNJ-38877605 for 12 h. After incubation, cells were reacted with monoclonal anti-a-tubulin antibody and Alexa Fluor 594-conjugated secondary antibody, DAPI was used for nuclear staining, the cellular microtubules were observed under fluorescence microscope. b MHCC97L and Huh7 cells were treated with tivantinib or SB216763 (a known GSK3 inhibitor) for 24 h. c Microtubule polymerization assay. Microtubules were incubated at 37°Cfor 1 h in the presence of tivantinib, paclitaxel (a microtubule polymer stabilizer), vincristine (a microtubule depolymerizer) or JNJ-38877605. Increased fluorescence indicates polymerization of microtubules. Scale bar, 20 μm