Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 12;15:245. doi: 10.1186/s12870-015-0633-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Matzke and Matzke. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — Comparison of Case12 and ArcLight responses to eATP. Top: MiCAM images of root tips of plants expressing ArcLight and Case12 with colored circles indicating the root and background regions used for the graphs. The images correspond to the beginning of the experiment (0 s), addition of ATP (100 s), highest response (145 s, Case12, increase of fluorescence; 205 s ArcLight, decrease of fluorescence) and recovery (846 s Case12; 920 s ArcLight), which can also be seen in the open black circles on the traces. Bottom: MiCAM raw data files were imported into Metamorph and combined into one stack for comparison of fluorescence intensity changes. The traces derived from the colored circled areas at the top are displayed over a time period of 1092 s. Either 2 mM ATP or buffer was added at approximately 100 sec as indicated by the blue arrow. The red and green traces represent the responses of ArcLight and Case12, respectively, to eATP addition. Pink and gold traces show the corresponding backgrounds for ArcLight and Case12, respectively. Turquoise and blue traces show the buffer controls for ArcLight and Case12, respectively. Dark red and dark green traces indicate background for buffer controls for ArcLight and Case12, respectively (MiCAM images not shown)