Fig. 4.

Inducing calcification in 4T1 cells with ascorbic acid and β-glycerophosphate. 4T1 cells were incubated in normal media (control) and an osteogenic cocktail (OC). a The cell cultures were stained with AR-S to detect calcium nodules and were photographed at the original magnification and at ×100 magnification. b The cell cultures were stained with cetylpyridinium chloride and were quantified by measuring the absorbance at 562 nm. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001