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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Methods Mol Biol. 2013;1078:55–63. doi: 10.1007/978-1-62703-640-5_6

Fig. 1.

Fig. 1

Representative phase-contrast (a) and immunofluorescent (b) micrographs of primary enteric neurons cultured from small intestinal myenteric plexuses of 8-week-old mouse. After 5 days in culture, cells were fixed with 4 % paraformaldehyde/PBS for 10 min and standard immunocytochemistry was performed using rabbit anti-Tuj-1 polyclonal antibody (1:5,000, Sigma) and Alexa Fluor® 488 (green) conjugated donkey anti-rabbit secondary antibody (1:400; Invitrogen). Hoechst 33258 was used for counterstaining of nuclei (Blue)