|
| |
| DMEM/F12 medium | 39.5 ml |
|
| |
| Chick Embryo Extract (CEE) | 7.5 ml |
|
| |
| Penn/Strep (100×) | 0.5 ml |
|
| |
| Gentamicin (500×) | 100 μl |
|
| |
| Amphotericin (100×) | 0.5 ml |
|
| |
| N2 (100×) | 0.5 ml |
|
| |
| B27 (50×) | 1 ml |
|
| |
| Glutamine (100×) | 0.5 ml |
|
| |
| FGF-b (10 μg/ml) | 50 μl |
|
| |
| EGF (10 μg/ml) | 100 μl |
|
| |
| Heparin (0.2 %) | 5 μl |
|
| |
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