|
| |
| DMEM/F12 medium | 44 ml |
|
| |
| Heat inactivated Fetal Bovine Serum (HS-FBS) | 1 ml |
|
| |
| Chick Embryo Extract | 2.5 ml |
|
| |
| Penn/Strep (100×) | 0.5 ml |
|
| |
| Gentamicin (500×) | 100 μl |
|
| |
| Amphotericin (100×) | 0.5 ml |
|
| |
| N2 (100×) | 0.5 ml |
|
| |
| B27 (50×) | 1 ml |
|
| |
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