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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Apr 13;35(3):366–376. doi: 10.1038/onc.2015.91

Figure 6. Requirement for Runx2-S301, S319 phosphorylation in stimulation of in vivo tumor growth.

Figure 6

PC3-luc cells were transduced with LacZ, Runx2-WT or Runx2-SA adenovirus vectors, trypsinized, suspended in MatrigelTM and subcutaneously implanted into immunodeficient mice. Equal levels of wild type and mutant Runx2 protein expression were verified on Western blots (B). Values below each lane indicate the Runx2/Gapdh protein ratio as determined by densitometry. PC3-luc cells were detected after 16 d by in vivo bioluminescence imaging (A, C). Mice were sacrificed at d 17. Tumors were photographed (D), weighed (E) and analyzed for Vegf (F), Mmp9 (G) or Spp-1 (H) mRNA normalized to 18S rRNA. Statistics: Panels C & E, aSignificantly different from LacZ control (p < 0.05), bSignificantly different from Runx2-WT (p < 0.01); Panels F-H, aSignificantly different from LacZ control (p < 0.05), bSignificantly different from Runx2-WT (p < 0.01), N = 6/group.