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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Apr 13;35(4):459–467. doi: 10.1038/onc.2015.97

Fig. 1. Knockdown of B-Raf expression in B-Raf mutant cancer cell lines reduces DR5 expression (A) and attenuates cell responses to TRAIL-induced apoptosis (B and C).

Fig. 1

BCPAP or LOXIMVI cells were transfected with the indicated siRNAs. After 62 h, the cells were harvested for preparation of whole-cell protein lysates and subsequent Western blotting to detect the indicated proteins (A). The cells were also exposed to 25 ng/ml TRAIL (B) or different concentrations of TRAIL (C) for an additional 8 h. Protein cleavage in whole-cell protein lysates prepared from these cells was detected with Western blotting (B). Viable cell numbers were estimated with the SRB assay (C). The data are means ± SDs of four replicate determinations. CF, cleaved form. ** P < 0.001; *** P < 0.0001 between two transfected cells exposed to every tested concentration.