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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Apr 13;35(4):459–467. doi: 10.1038/onc.2015.97

Fig. 6. The combination of PLX4032 and AZD6244 is as effective as PLX0432 or AZD6244 alone in suppressing DR5 expression (A) and attenuating TRAIL-induced apoptosis (B and C).

Fig. 6

A, The indicated cancer cell lines were exposed to the given concentrations of the tested agent alone or their combination. After 16 h, the cells were harvested for preparation of whole-cell protein lysates and subsequent detection of the indicated proteins with Western blotting. B and C, A375 cells were pretreated with 5 μM PLX4032, AZD6244 or their combination for 12 h. After washing 3 times with the medium, the cells were re-fed with medium containing 100 ng/ml for additional 4 h. Protein cleavage was then detected with Western blotting (B) and DNA fragments were evaluated with the Cell Death Detection ELISAPlus kit (C) from these cells. The data are means ± SDs of triplicate determinations (C). CF, cleaved form. *** P < 0.0001 in comparison with TRAIL alone treatment.