Figure 1.
Immunofluorescent staining of aortic valve interstitial cells (AVICs) for matrix glaprotein (MGP). MGP and the nuclei are stained green and blue, respectively. White arrows denote cells staining positive for MGP. Normal AVICs were stained for MGP and images were taken at high (A) and low (B) power. Normal AVICs were also stained with IgG as a control at high (C) and low (D) power. Diseased AVICs were stained with MGP and imaged at high (E) and low (F) power. Diseased cells were also stained with control IgG at high (G) and low (H) power. Disease AVICs express lower levels of MGP relative to normal AVICs. Scale bar = 50μm.